Fig. 2 Effect of TTO, terpinen- 4-ol (T4OL) and alpha-terpineol (alpha) on TLR2/4 and TLR4- induced cytokine production by macrophages.

Fig. 2 Effect of TTO, terpinen-4-ol (T4OL) and alpha-terpineol (alpha) on TLR2/4 and TLR4-induced cytokine production by macrophages. The oils were added at the indicated concentrations 2 h prior to stimulation with LPS from E.coli 1 lg/mL (a, c, e, g) or P.gingivalis 1 lg/mL (b, d, f, h). Stimulation with LPS was carried out for 24 h. Cytokine production was measured in cell
culture supernatants by multiplex analysis (in pg/mL). Asterisk a significant difference (t test for unpaired samples) in comparison with LPS stimulation alone (no pretreatment with any oil)(p.05)